SubtiBank SubtiBank
ydaB [2017-10-10 09:20:06]
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ydaB [2017-10-10 09:20:06]

similar to acyl-CoA synthetase (long-chain-fatty-acid--CoA ligase)
locus
BSU04170
pI
4.85
mw
51.07 kDa
protein length
465 aa Sequence Blast
gene length
1395 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    469,426 → 470,937

    The protein

    Protein family

  • ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

    • Structure

  • [PDB|3R44] (from Mycobacterium tuberculosis, 33% identity) [pubmed|22560731]

    • Expression and Regulation

    Operons

    genes
    [gene|2436420C7781DCB61B9A939B47747265E23740A5|ydaB]
    description
    [Pubmed|21815947]

    additional information

  • the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] (the half-life of the mRNA increases from 2 to 80 min) [PubMed|21815947]
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    Biological materials

    Mutant

  • BKE04170 (Δ[gene|2436420C7781DCB61B9A939B47747265E23740A5|ydaB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGTCATCTCCTATAT, downstream forward: _UP4_TCATAAAAGAAGAAGCCCGT
  • BKK04170 (Δ[gene|2436420C7781DCB61B9A939B47747265E23740A5|ydaB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGTCATCTCCTATAT, downstream forward: _UP4_TCATAAAAGAAGAAGCCCGT

    • References

  • 1460045,21815947,22560731